中国油料作物学报2013，Vol.35Issue(1)：74-79,6.
酸性土壤中大豆根际微生物群体感应的初步研究
Initial study on quorum sensing of rhizosphere microbes of soybeans in acid soil
摘要
Abstract
Bacterial quorum sensing ( QS) in the rhizosphere soil of soybean in acid soil was studied with pot and laboratory experiments. N - acyl - homoserine lactone ( AHL) was one of QS signal molecules, it was extracted from the rhizosphere and bulk soils of aluminum (Al) -tolerant (BX10) and Al -sensitive (BD2) soybean genotypes cultured in acid soil ,and was detected by Agrobacterium tumefaciens KYC55 (pJZ372; pJZ384; pJZ410). The results showed that the AHL can be detected in the rhizosphere soil of soybeans; the AHLs activities in the rhizosphere soil were much higher than those in the bulk soil; the AHLs activities in the rhizosphere soil of BX10 were much higher than those of BD2 (P <0. 01). Moreover, AHLs increased gradually during the process of soybean growth. The results of the laboratory experiments simulating the acid soil environment showed that different concentrations of Al significantly influenced the growth, the AHLs level, the production of exopolysaccharide and root hair adherence of Sinorhizobium sp. 1128, which further demonstrated the effects of aluminum stress on QS. This study facilitates the further studies about the molecular mechanism of interaction between plant and rhizosphere microbes in acid soils.关键词
大豆/酸性土壤/铝毒/根际微生物/群体感应/AHLKey words
Soybean/ Acid soil/ Aluminum toxicity/ Rhizosphere microbial/ Quorum sensing/ AHL分类
生物科学引用本文复制引用
刘玥,华小梅,郭靖,扈冬青,朱敏敏,杨统一,李永春,戚金亮,杨永华..酸性土壤中大豆根际微生物群体感应的初步研究[J].中国油料作物学报,2013,35(1):74-79,6.基金项目
国家自然科学基金(30971871,40771107) （30971871,40771107）
教育部创新团队项目(IRT1020) （IRT1020）
教育部高校科技创新工程重大项目培育资金(707027) （707027）
