安徽农业科学2013，Vol.41Issue(10)：4255-4258,4267,5.
重金属结合蛋白基因hmbp在转基因拟南芥中的功能分析
Functional Analysis of Heavy Metal Binding Protein Gene hmbp in Transgenic Arabidopsis
摘要
Abstract
The paper was to analyze the function of heavy metal binding protein gene hmbp in transgenic Arabidopsis.[Method] The hmbp gene was cloned from Deinococcus radiodurans by the PCR method and was transferred into Arabidopsis by the means of floral dip method.The positive plants were obtained after cadmium chloride treatment,and the MDA content,proline content,soluble sugar content,chlorophyll content,relative conductivity,SOD activity,POD activity of transgenic plants were detected.[Result] Compared with the wild type,the transgenic Arabidopsis had more rise of proline content,soluble sugar content,SOD activity and POD activity of thaliana after CdCl2 treatment,with less rise of MDA content and less abatement of chlorophyll content.[Conclusion] The study showed that the hmbp transgenic Arabidopsis had high resistance to cadmium.关键词
植物修复/镉/转基因拟南芥/hmbpKey words
Phytoremediation/ Cadmium / Transgenic Arabidopsis/ hmbp分类
农业科技引用本文复制引用
周惠,辛培尧,赵丽华,原晓龙,陈杰,周军..重金属结合蛋白基因hmbp在转基因拟南芥中的功能分析[J].安徽农业科学,2013,41(10):4255-4258,4267,5.基金项目
上海市科技计划项目(CIE01G1106)资助. （CIE01G1106）
