安徽农业科学2013，Vol.41Issue(10)：4629-4630,4635,3.
比色法测定稻米γ-氨基丁酸含量的体系优化
Optimization of Determination γ-Aminobutyric Acid in Brown Rice by Colorimetric Method
摘要
Abstract
To optimize method for determining γ-aminobutyric acid content in rice by colorimetric method and establish a simple,rapid and accurate brown rice GABA determination system.[Method] Based on Berthlot chromogenic reaction,determination of γ-aminobutyric acid content in rice by colorimetric method was optimized from determination wavelength,time,phenol,sodium hypochlorite and brown rice dosage.[Result] Theoptimum conditions were:wavelength 630 nm,5％ phenol solution 3.4 ml,available chlorine 7％ sodium hypochlorite solution 1.5 ml,determination time 60-180 min.the optimum rice dosage 1.2 ml.[Conclusion] The system is simple,rapid,economy and accurate in determining γ-aminobutyric acid content in rice,which can provide reference for screening and breeding GABA enrichment rice variety.关键词
γ-氨基丁酸/比色/糙米Key words
γ-aminobutyric acid / Colorimetric / Brown rice分类
农业科技引用本文复制引用
韩延丽,谭学林,谭亚玲..比色法测定稻米γ-氨基丁酸含量的体系优化[J].安徽农业科学,2013,41(10):4629-4630,4635,3.基金项目
云南省杂交粳稻工程技术研究中心(2011DH016) （2011DH016）
云南省教育厅项目(2010C249) （2010C249）
云南省科技厅水稻产业体系(A3006923). （A3006923）
