安徽农业科学2013，Vol.41Issue(11)：4701-4703,4737,4.
白腐菌产漆酶培养条件的响应面法优化
Optimization of Laccase Production by Coprinopsis cinerea Using Response Surface Methodology
摘要
Abstract
[Objective] Process parameters for laccase production by Coprinopsis cinerea were optimized based on Plackett-Burman experimental design and response surface analysis.[Method] Important factors affecting laccase production,which were identified by initial screening method of Plackett-Burman,were yeast extract,straw powder and wheat bran concentration.Path of steepest ascent was undertaken to approach the optimal region of the significant factors.Box-Behnken design and response surface analysis were adopted to further investigate mutual interaction between variables.[Result] Yeast extract,straw powder and wheat bran concentration were significantly correlated with laccase production.Laccase production was up to 729.89 U/ml at the optimal conditions of yeast extract concentration 3.613 g/L,straw powder concentration 9.379 g/L and wheat bran concentration 10.188 g/L.[Conclusion] After 3 times of cultivating verificataion,predication value and test value were close.关键词
Coprinopsis cinerea/漆酶/培养条件/响应面法Key words
Coprinopsis cinerea /Laccase / Culture condition/ Response surface methodology分类
农业科技引用本文复制引用
毕云枫,李振光,李彦阳,刘薇薇,沈明浩..白腐菌产漆酶培养条件的响应面法优化[J].安徽农业科学,2013,41(11):4701-4703,4737,4.基金项目
吉林省教育厅项目[吉教科合字(2011)第35号]. （2011）
