安徽农业科学2013，Vol.41Issue(11)：4729-4734,6.
基于硒蛋白生物合成机制的硒生物检测器
Biological Selenium Detector Based on Mechanism of Selenoprotein Biosynthesis in Prokaryotes
摘要
Abstract
Selenium,one trace essential elements for many organisms,is crucial to human health.Imbalance of selenium distribution in nature would result the marked variation in local environmental selenium levels which would lead to the selenosis or selenium deficiency.The elucidation of pathways for biosynthesis of selenocysteine (Sec) and selenoproteins in prokaryotes made it possible for the construction of biological selenium detector which would supplement the current selenium measuring methods.Selenocysteine insertion sequence (SECIS) is necessary for the Sec insertion,and its preceding nucleotides had been found influenced the efficiency for Sec insertion.By varying the preceding nucleotides,high accurate and efficient SECIS were screened based on the effects on translation of β-lactmase which activity was easily detected by bacterial anti-ampicillin activity.The best SECIS would be further used to direct translation of GFP reporter gene together with SelABC to quantitatively report selenate concentration.After induction with IPTG and incubation with selenate,fluorescence intensity of the cell lysate was found linearly correlated with selenate concentrations very well in our testing selenate concentrations.This linear correlation indicated that the constructed biological detector is successful so far and laid foundation for measuring selenate contents of environmental and biological samples with this detector in the future.关键词
硒蛋白/硒代半胱氨酸/生物检测器/茎环结构Key words
Selenoprotein / Selenocysteine / Biological detector/ Selenocysteine insertion sequence分类
农业科技引用本文复制引用
邱洋松,孙婧,孙爱,邬丹妮,张冰倩,马建辉,徐丽珊,孙梅好..基于硒蛋白生物合成机制的硒生物检测器[J].安徽农业科学,2013,41(11):4729-4734,6.基金项目
国家自然科学基金项目(No.30800019) （No.30800019）
浙江省自然科学基金项目(No.Y3090546) （No.Y3090546）
教育部留学归国科研启动基金. （）
