安徽农业科学2013，Vol.41Issue(11)：4740-4744,5.
六倍体普通小麦中国春PBF编码基因的克隆及序列分析
Cloning and Sequence Analysis of PBF Encoding Genes from Hexaploid Common Wheat Chinese Spring (Triticum aestivum L.)
摘要
Abstract
[Objective] This study aimed to investigate the polymorphism of PBF encoding genes from common wheat Chinese Spring (Triticum aestivum L.).[Method] Using common wheat Chinese Spring as the experimental material,gene-specific primers were designed and applied to amplify the genomic DNA of Chinese Spring.PCR products were isolated,purified and ligated into the cloning vector.Positive clones were randomly selected for sequencing.A series of softwares including DNAMAN,Signalp,PSIPRED,Nuc_PLoc and MEGA were employed for sequence assembly and alignment,signal peptide prediction,primary and secondary structure prediction,as well as analyses of subcellular location and phylogenetic relationships between the PBF family members in Gra mineae.[Result] Twenty-five target sequences were obtained from the genome of hexaploid common wheat Chinese Spring,which were classified into three clusters based on the sequence similarity.SNPs exist at two loci of the subunit,resulting in the change of encoded amino acid residues and affecting the secondary structure of final product encoded.[Conclusion] PBF encoding sequences are extremely conservative in Chinese Spring with certain variations.This study provides theoretical reference to evaluate the expression efficiency of wheat storage proteins.关键词
普通小麦/PBF转录因子/克隆/序列分析Key words
Common wheat/ PBF transcription factor/ Cloning/ Sequence analysis分类
农业科技引用本文复制引用
郭丽娜,高翔..六倍体普通小麦中国春PBF编码基因的克隆及序列分析[J].安徽农业科学,2013,41(11):4740-4744,5.基金项目
国家自然基金(30900896) （30900896）
现代农业产业技术体系建设专项(NYCYTX-001) （NYCYTX-001）
唐仲英育种基金(A212020912). （A212020912）
