安徽农业科学2013，Vol.41Issue(11)：4773-4776,4.
响应面法优化超临界CO2萃取当归挥发油工艺及抑菌活性研究
Optimization of Supercritical Carbon Dioxide Extraction Conditions of Angelica sinensis Volatile Oil Using Response Surface Method and the Detection Research of Antimicrobial Activity
金元宝 1杜志琳 2刘明石 1王莘1
作者信息
- 1. 吉林农业大学生命科学学院,吉林长春130118
- 2. 吉林大学珠海学院,广东珠海519041
- 折叠
摘要
Abstract
[Objective] Response surface analysis method was applied to optimize the supercritical carbon dioxide extraction conditions of Angelica sinensis,the antimicrobial activity of Angelica sinensi volatile oil was studied.[Method] Based on the single factor experiment,a three-factor-three-level experiment design was developed by Box-Benhken central composite design method.The influences of extraction temperature extraction time extraction pressure on the yeild of extraction were studied.The response surface method was employed to analyze the results of experiments.The disk diffusion method was used to detect the antimicrobial activity of Angelica sinensi volatile oil.[Result] The results indicated that the optimum extraction conditions were as follows:extraction temperature 44.4 ℃,extraction time 1.48h,extraction pressure 32.37 Mpa,the extraction yield reached 2.0833％.The volatile oil of Angelica sinensis had antimicrobial activity of the tested strains,the MIC of the volatile oil was ranged 1.25 mg/ml from 5 mg/ml.[Conclusion] The supercritical carbon dioxide extraction condition is a stability process to obtain a higher extraction yield.The Angelica sinensis volatile oil has antimicrobial activity which could provide certain theoretical basis for the application of volatile oil.关键词
超临界CO2萃取/当归油/响应面分析/抑菌活性Key words
Supercritical carbon dioxide/ Angelica oil/ Response surface method/ Antibacterial activity分类
农业科技引用本文复制引用
金元宝,杜志琳,刘明石,王莘..响应面法优化超临界CO2萃取当归挥发油工艺及抑菌活性研究[J].安徽农业科学,2013,41(11):4773-4776,4.
