安徽农业科学Issue(12)：5278-5279,2.
HPLC法测定大黄药材中5种游离蒽醌的含量
HPLC Method for the Determination of 5 Kinds of Free Anthraquinone in Rhubarb
摘要
Abstract
[Objective] The establishment of HPLC method for simultaneous determination of aloe emodin in rhubarb,rhein,emodin,chrysophanol physcion,content,and different places in rhubarb samples.[Method] ODS2 C18 column (200 mm ×4.6 mm,5 μm),mobilephaseof methanol:0.1％ phosphoric acid =75∶25 (V/V),the flow rate was 1 ml/min,the detection wavelength was 254 nm,column temperature was 25 ℃.[Result] The linear regression equation of aloe-emodin; rhein; emodin; chrysophanol ; physcion are Y =1.96X-0.25,correlation coefficient R =0.999 94 ; Y =2.45 X + 0.18,R =0.999 94; Y =1.91 X + 0.05,R =0.999 90 ; Y =1.25 X-0.06,R =0.999 95 ; Y =0.96 X-0.20,R =0.999 98 ; the average recoveries were 99.39％,98.84％,98.94％,99.11％,98.37％.[Conclusion] The method is simple,rapid,reproducible,accurate results,and can be used for quality evaluation and quality test of rhubarb.关键词
HPLC/大黄/芦荟大黄素/大黄酸/大黄素/大黄酚/大黄素甲醚Key words
HPLC / Rhubarb/ Aloe-emodin/ Rhein / Emodin / Chrysophanol/ Physcion分类
农业科技引用本文复制引用
李红英,向极钎,龙澜,帅超群,李亚杰..HPLC法测定大黄药材中5种游离蒽醌的含量[J].安徽农业科学,2013,(12):5278-5279,2.基金项目
湖北省科技计划研究与开发项目(2009BHB001). （2009BHB001）
