安徽农业科学Issue(12)：5502-5503,5509,3.
微生物法测定维生素饮料中的维生素B6
Microbiological Method for Assay of Vitamin B6 in Vitamin Drinks
陈亚波 1周敏 1杨彤1
作者信息
- 1. 中山市质量计量监督检测所,广东省质量监督食品检验站(中山),广东中山528403
- 折叠
摘要
Abstract
[Objective] To establish a microbiological method for assay of vitamin B6 in vitamin drinks.[Method] The Saccharomyces Carlsbrgensis (ATCC 9080) has great sensitivity for vitamin B6 in materials.According to this specificity,it can be assay vitamin B6 in samples.Prepare standard concentrate-response curve by plotting absorbance reading for each level of standard solution.Determine the amount of vitamin B6 activity for each level of assay solution by interpolating from standard curve.[Result] The precision were 1.5％,1.1％ and 1.5％.The RSD was 0.788 7％.The recoveries were all above 96.5％.[Conclusion] Microbiological method can be used to determination of vitamin B12 in vitamin drinks and this method has high sensitivity.The results are precise.关键词
微生物法/维生素B6/卡尔斯伯酵母菌Key words
Microbiological method/ Vitamin B6/ Saccharomyces Carlsbrgensis分类
农业科技引用本文复制引用
陈亚波,周敏,杨彤..微生物法测定维生素饮料中的维生素B6[J].安徽农业科学,2013,(12):5502-5503,5509,3.
