安徽医科大学学报2013，Vol.48Issue(5)：458-462,5.
rhBMP-2联合rhVEGF165对兔前交叉韧带重建后腱-骨愈合的影响
Effects of administration rhBMP-2/rhVEGF165 on tendon-bone healing after anterior cruciate ligament reconstruction in rabbits
摘要
Abstract
Objective To explore effects of local administration rhBMP-2/rhVEGF165 on tendon-bone ealy healing after anterior cruciate ligament reconsctruction in rabbits. Methods Seventy eight male New Zealand white rabbits were randomly divided into Group VB,B,V,F and normal group. Bilateral anterior cruciate ligaments of each animal were removed and reconstructed with autogenetic tendon of semitendinosus muscles while normal group did not receive any operation. The fibrin glue ( FG ) containing rhBMP-2/rhVEGF165 was injected into tendon-bone interface of Group VB. FG with rhBMP-2 was injected in Group B, FG with rhVEGF165 in Group V and only FG were used in Group F. Six animals were sacrificed randomly in each group at 2th, 4th, 8th posteoperative week respectively for right knee specimens histological examinations and left knee specimens biomechanical tests. Results After two weeks of operation, the tendon and bone were connected tightly with active proliferation of fibroblast at Group VB; some chondrocytes were observed in the tight interface of Group B; the interfaces of Group V and F consisted primarily of fibrovascular tissue with loose connection. After four weeks of operation, the interface of Group VB initially formed four-layer insertion, while those of Group B, V, and F composed mainly of mature fibro-blasts with tight connection and a certain osteogenetic reaction was observed in the bone tunnel wall. After eight weeks of operation, there was more obvious four-layer insertion with tidemark in the interface of Group VB and osteogenetic reaction further progressed in the bone tunnel wall in Group B. The width of tendon-bone interface of Group V and F narrowed further and the number of fibroblasts significantly reduced. Not unitl 4 and 8 weeks after operation, were the average maximum load and stiffness of Group VB higher than thoses of Group B, V, and F ( P <0. 05 ). Meanwhile, the biomechanical property of Group V and B were better than those of Group F ( P < 0. 05 ). Conclusion Combined administration rhBMP-2 and rhVEGF165 can enhance tendon-bone early healing after anterior cruciate ligament reconstruction in rabbits and the effects of combination are stronger than that of single use.关键词
前交叉韧带/骨形态发生蛋白/血管内皮生长因子/腱-骨愈合/重建Key words
anterior cruciate ligament/ BMP-2/ VEGF165/ tendon-bone healing/ reconstruction分类
医药卫生引用本文复制引用
周平,赵其纯,尚希福,王姚斐,李旭,纪小枫,凌晓冬,朱亚林..rhBMP-2联合rhVEGF165对兔前交叉韧带重建后腱-骨愈合的影响[J].安徽医科大学学报,2013,48(5):458-462,5.基金项目
安徽省科技厅重点科研项目(编号:11070403044) （编号:11070403044）
