安徽医科大学学报2013，Vol.48Issue(5)：561-564,4.
羟基磷灰石/壳聚糖/聚乳酸骨组织工程支架材料生物相容性研究
Biocompatible research of hydroxyapatite/chitosan/poly lactic acid as material of stent in bone tissue project
摘要
Abstract
Hydroxyapatite/ohitosan/poly( L-lactio acid ) ternary nanocomposites scaffold was prepared by in situ method and solution blending method. In vitro 293T cells were encapsulated into the scaffold and cultivated in three-dimensional envirnment to observe the biocompatibility of the scaffolds including cell viability, adhesion, proliferation by inverted microscope and SEM. The cell proliferation was tested by MTT method and physical performance was tested by physical method. Live/dead staining with calcein acetoxymethyl ester/Ethidium homodimer-1 ( Calcein-AM/EthD-1 ) was performed in order to determine cell viability on the scaffold. The results showed that hydroxyapatite/chitosan/poly ternary nano biocomposites scaffold display high mechanical property, hydrophilicity and good biocompatibility, suggesting the potential of this composites for applications in bone tissue engineering.关键词
羟基磷灰石/壳聚糖/聚乳酸/骨组织工程/支架材料Key words
hydroxyapatite/ chitosan/ poly( L-lactic acid )/ bone tissue engineering/ scaffolds分类
医药卫生引用本文复制引用
吕洪磊,张彩云,顾玉芳,王云,赵宇..羟基磷灰石/壳聚糖/聚乳酸骨组织工程支架材料生物相容性研究[J].安徽医科大学学报,2013,48(5):561-564,4.基金项目
安徽省教育厅自然科学基金重大项目(编号:KJ2011ZD04、KJ2012ZD09) （编号:KJ2011ZD04、KJ2012ZD09）
国家自然科学基金(编号:81171829) （编号:81171829）
安徽省科技厅自然科学基金(编号:10040606Q36) （编号:10040606Q36）
