安徽医科大学学报2013，Vol.48Issue(6)：595-600,6.
肾小管上皮-间质细胞转分化模型的建立
Establishment of a model of epithelial-mesenchymal transdifferentiation in HK-2 cells
摘要
Abstract
Objective To investigate the roles of transforming growth factor β1(TGF-β1) during transdifferentiation of human renal tubular epithelial cells, and to establish a model of epithelial-mesenchymal transdifferentiation. Methods The experiment was divided into control and experimental groups. Human renal tubular epithelial cells ( HK-2 ) were treated by TGF-β1 for 24 hours. The molecular markers in epithelial or mesenchymal cells were investigated by using Western blot and immune-fluorescence. To determine migration and invasion, scratching test and transwell assay were performed. Results The mesenchymal morphology was exhibited in HK-2 cells treated for 24 h by TGF-β1( 10 ng/ml ). Experiment data also showed that TGF-β1 can increase the expression of the mesenchymal phenotype markers ( Vimentin, β-catenin ), and repress the expression of the epithelial phenotype marker ( E-cadherin ) in HK-2 cells. At the same time, compared with the control and the TGF-β1 groups, scratching test showed that TGF-β1 promoted HK-2 cell migration. The difference was statistically significant ( P < 0. 01 ). In terms of invasion, a statistically significant difference was observed between the control group and TGF-β1 group ( P <0. 01 ). Conclusion Taken together, our findings provide the cell model of EMT in vitro using TGF-β1.关键词
肾间质纤维化/肾小管上皮细胞/转分化/转化生长因子β1Key words
renal interstitial fibrosis/ renal tubular epithelial cell/ transdifferentiation/ transforming growth factor β1分类
医药卫生引用本文复制引用
陈芳,成芳,项倩彤,郭思佳,查晓军,王德光,周海胜..肾小管上皮-间质细胞转分化模型的建立[J].安徽医科大学学报,2013,48(6):595-600,6.基金项目
国家自然科学基金(编号:81172591) （编号:81172591）
