安徽医科大学学报2013，Vol.48Issue(7)：734-738,5.
CARex和PTDtat基因重组5型腺病毒的包装及鉴定
Package of recombinant adenovirus serotype 5 carrying with CARex and PTDtat gene
摘要
Abstract
Objective To construct and pack the recombinant adenovirus serotype 5 carrying CARex, PTDtat, CARex-PTDtat gene respectively. Methods The recombinant shuttle plasmid pAdtrack-CMV-CARex, pAdtrack-CMV-PTDtat,pAdtrack-CMV-CARex-PTDtat were constructed successfully by vitro ligation, the recombinant shuttle plasmid were linearized by Pme I and then transformed to BJ5183 cell containing the pAdEasy-1 bone plasmid and the conformed plasmid were tansformed to DH5α cells for larger scale preparation of this plasmid, pu-rifing recombinant adenovirus plasmid digested with Pac I, then transfected 293 A cells using liposome. Results Recombinant adenovirus serotype 5 carrying CARex,PTDtat,CARex-PTDtat gene were packaged in 293A cells, and identified successfully by inverted fluorescence microscope and PCR. Conclusion Construction and package of the recombinant adenovirus pAd-CARex, pAd-PTDtat, pAd-CARex-PTDtat for preparation of infection the tumor cells and testing the function of CARex and PTDtat gene in the adenovirus infections.关键词
CARex/PTDtat/同源重组/重组腺病毒载体Key words
CARex/ PTDtat/ recombinant/ recombinant adenovirus分类
医药卫生引用本文复制引用
陈红霞,赵前锋,周海胜,刘淼,任翠平,沈际佳..CARex和PTDtat基因重组5型腺病毒的包装及鉴定[J].安徽医科大学学报,2013,48(7):734-738,5.基金项目
国家自然科学基金(编号:30771882、81271815) （编号:30771882、81271815）
