安徽医科大学学报2013，Vol.48Issue(7)：764-768,5.
不同信号肽对重组胰高血糖素样肽-1载体表达效率的影响
Effects of different signal peptides on secretion of recombinant GLP-1
吴冬 1刘尚全 1袁媛 2张维娜 2李菲1
作者信息
- 1. 安徽医科大学第三附属医院内分泌科,合肥230000
- 2. 合肥市滨湖医院中心实验室,合肥230601
- 折叠
摘要
Abstract
Objective To explore the effects of three types of signal peptides on recombinant GLP-1 secretion in development of screening optimal expression vector of GLP-1. Methods The recombinant expression vectors of GFP-GCG-GLP-1 , GFP-NT4-GLP-1 , GFP-EX4-GLP-1 were constructed and transfected in HEK293T cell with Vigofect. The mRNA expression of GLP-1 was analysed by RT-PCR in HEK293T cell and the protein secretion of GLP-1 was tested by ELISA in the cells supernatant. Results RT-PCR results show that there were no significant differences in the expression levels of GLP-1 among the constructs at 24 h after transfection. The GLP-1 protein secretion of EX4 group was significantly higher than other group at 24 h after transfection ( P <0. 01 ). Conclusion The EX4 signal sequence could be effective on promoting the secretion of other heterologous proteins in HEK293T cells.关键词
信号肽/GLP-1/基因表达Key words
signal peptide / GLP-1 / gene expression分类
医药卫生引用本文复制引用
吴冬,刘尚全,袁媛,张维娜,李菲..不同信号肽对重组胰高血糖素样肽-1载体表达效率的影响[J].安徽医科大学学报,2013,48(7):764-768,5.
