安徽医科大学学报2013，Vol.48Issue(7)：790-793,4.
胃癌患者外周血及癌组织中CD4+CD25+调节性T细胞、转录因子Foxp3的表达及临床意义
Expression and clinical signification of CD4+CD25+ regulatory T cells,the transcription factor Foxp3 in peripheral blood and cancerous tissue of gastric cancer
摘要
Abstract
Objective To investigate the expression and clinical signification of CD4 + CD25 + regulatory T cells ( Tregs ), Foxp3 in peripheral blood and cancerous tissue of gastric cancer. Methods Preoperative blood sample of 38 patients with gastric cancer and 20 cases of healthy control group were subjected to flow cytometry. Gastric cancer tissue and para-tumorous tissue ( from the tumor edge > 5 cm ) from gastric cancer were subjected to immunohis-tochemistry to detect CD4 + CD25 + Foxp3 + Tregs expression, and the correlation analysis with some clinical related parameters was made. Results The percentages of CD4 + CD25 + Foxp3 + Tregs in peripheral blood of gastric cancer group were significantly higher than those from healthy control group( P <0. 01 ),and were highly correlated to TNM stage, lymph node metastases( P <0. 01 ). The percentages of Foxp3 + Tregs in gastric cancer tissue were significantly higher than those from para-tumorous tissue( P <0. 01 ),and were highly correlated to TNM stage,tumor differentiation,lymph node metastases( P < 0. 01 , P < 0. 05 ). The percentages of CD4 + CD25 + Foxp3 + Tregs in peripheral blood was positively correlated to Foxp3 + Tregs in gastric cancer tissue( r - 0. 786, P < 0. 01 ). Conclusion The proportion of CD4 + CD25 + Foxp3 + Tregs in Peripheral blood and Foxp3 + Tregs in gastric cancer tissue is significantly elevated in human gastric cancer, and correlated with the genesis and development of gastric cancer, which can provide the theory basis for the early diagnosis and immunotherapy of gastric cancer.关键词
胃癌/CD4+CD25+调节性T细胞/Foxp3/流式细胞术/免疫组化Key words
gastric cancer/ CD4 + CD25 + Tregs/ Foxp3 / FCM / IHC分类
医药卫生引用本文复制引用
陆威,李永翔,张尚鑫,闫强..胃癌患者外周血及癌组织中CD4+CD25+调节性T细胞、转录因子Foxp3的表达及临床意义[J].安徽医科大学学报,2013,48(7):790-793,4.基金项目
国家自然科学基金(编号:30871207) （编号:30871207）
安徽高校省级自然科学研究重点项目(编号:KJ2008A154) （编号:KJ2008A154）
安徽省科技厅重点科研项目(编号:12070403086) （编号:12070403086）
