安徽中医学院学报2013，Vol.32Issue(3)：85-87,3.DOI:10.3969/j.issn.1000-2219.2013.03.027
高效液相色谱法测定山茱萸中熊果酸与齐墩果酸的含量
Content Determination of Ursolic Acid and Oleanolic Acid in Comus Officinalis by High Performance Liquid Chromatography
刘毅 1金传山 2许甜甜3
作者信息
- 1. 安徽中医药大学研究生部,安徽,合肥,230038
- 2. 安庆医药高等专科学校,安徽,安庆,246052
- 3. 安徽中医药大学药学院,安徽,合肥,230031
- 折叠
摘要
Abstract
Objective To determine the content of ursolic acid and oleanolic acid in Comus of ficinalis by high performance liquid chromatography (HPLC) and to provide a basis for the quality control of Comus of ficinalis. Methods HPLC was performed on a Diamonsil C18 column (4. 6 mm×250 mm, 5μm) at a column temperature of 25℃ , a flow rate of 1. 0 ml/min, a sample size of 20 μl, and a detection wavelength of 210 nm. The mobile phase used in HPLC consisted of water and a mixture (composed of water, glacial acetic acid, and triethylamine at a ratio of 30:0. 1: 0. 05) at a ratio of 88: 12. Results Ursolic acid and oleanolic acid had a good linear relationship between concentration and peak area in the concentration ranges of 0. 031 5-0. 315 mg/ml and 0. 015 6-0. 156 mg/ml, respectively; the average recovery rates of ursolic acid and oleanolic acid were 97. 53% (RSD = 1. 28%) and 100. 08% (RSD = 1. 98%) , respectively. Conclusion This HPLC method is easy to operate, highly repeatable, accurate, and reliable and can be used for the quality control of Comus officinalis.关键词
山茱萸/熊果酸/齐墩果酸/高效液相色谱法Key words
Com us officinalis / ursolic acid/ oleanolic acid/high performance liquid chromatography分类
医药卫生引用本文复制引用
刘毅,金传山,许甜甜..高效液相色谱法测定山茱萸中熊果酸与齐墩果酸的含量[J].安徽中医学院学报,2013,32(3):85-87,3.
