北京林业大学学报2013，Vol.35Issue(4)：144-148,5.
适用于毛白杨芽双向电泳分析的蛋白质提取方法
Optimal protein extracting methods of Populus tomentosa buds for two-dimensional gel electrophoresis
摘要
Abstract
Three protein extracting methods (Tris-phenol method,TCA/acetone method and TCA/acetone +Tris-phenol method) were compared with the materials of P.tomentosa buds and described in this report.To determine which protein extracting method is most suitable for P.tomentosa buds,two-dimensional electrophoresis was applied to the separation of proteins,PDQuest software and mass spectrometry were used to characterize the protein spots from 2D-gel.Among the three protein extracting methods,TCA/acetone method was the most suitable one for P.tomentosa buds and yielded (3.57 ±0.618) mg/g total protein amount in fresh sample,231 ±4 protein spots on the 2D-Gel and the clearest 2D-Gel images,which were higher in total amount,more in spot number and clearer in 2D-Gel image than other two methods.关键词
毛白杨芽/蛋白质提取/双向电泳/质谱Key words
Populus tomentosa buds/ protein extraction/ two-dimensional gel electrophoresis/ mass spectrometry分类
农业科技引用本文复制引用
谢进,田晓明,刘淑欣,侯佳音,盖颖,蒋湘宁..适用于毛白杨芽双向电泳分析的蛋白质提取方法[J].北京林业大学学报,2013,35(4):144-148,5.基金项目
"863"国家高技术研究发展计划项目(2011AA100203)、国家自然科学基金项目(NSF30630053).学与技术学院. （2011AA100203）
