北京中医药大学学报2013，Vol.36Issue(5)：322-326,后插2,6.DOI:10.3969/j.issn.1006-2157.2013.05.009
芪蛭益肺颗粒对慢性阻塞性肺疾病模型大鼠小气道上皮MMP-9、TIMP-1表达的影响
Influences of Qizhi Yifei Granules on expressions of MMP-9 and TIMP-1 in small airway epithelium in rats with chronic obstructive pulmonary disease
摘要
Abstract
Objective To investigate the effect of Qizhi Yifei Granules on remodeling of small airway and expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in rats with chronic obstructive pulmonary disease (COPD).Methods All rats (n =72) were randomly divided into normal group,model group and treatment group.The rat model of COPD was established by using the method combining smoking fuming and intratracheal LPS instillation.From the 21 st day,model group was intragastrically given saline once a day,treatment group,Qizhi Yifei Granules once a day and blank group,normal feeding.All rats were killed on the 30th day,40th day and 60th day in batches.The expressions of MMP-9 and TIMP-1 were detected by using immunohistochemistry technique.Results In model group,the expression of MMP-9 was lower on the 30th day and higher on the 60th day compared with normal group (P ＜ 0.05).The expression of MMP-9 had no statistical difference in treatment group compared with model group at all time points.In model group,the expression of TIMP-1 had no difference compared with normal group at all time points,and decreased in treatment group on the 30th day (P ＜ 0.05).The ratio of MMP-9 to TIMP-1 decreased in model group compared with normal group on the 30th day and 40th day,and increased in treatment group compared with model group (P ＜ 0.05).Conclusion Qizhi Yifei Granules can intervene the remodeling of small airway in rats with COPD through up-regulating the ratio of MMP-9 to TIMP-1 before 40 days.关键词
慢性阻塞性肺疾病/小气道重塑/信号通路/动物模型/芪蛭益肺颗粒/大鼠Key words
chronic obstructive pulmonary disease/small airway remodeling/signal pathway/animal model/Qizhi Yifei Granules/rats分类
医药卫生引用本文复制引用
刘治坤,张前,李宝萍,于会勇,李根茂,葛东宇,黄文通,冯淬灵..芪蛭益肺颗粒对慢性阻塞性肺疾病模型大鼠小气道上皮MMP-9、TIMP-1表达的影响[J].北京中医药大学学报,2013,36(5):322-326,后插2,6.基金项目
国家自然科学基金资助项目(No.81072769) （No.81072769）
