重庆医学2013，Vol.42Issue(17)：1932-1934,3.DOI:10.3969/j.issn.1671-8348.2013.17.002
人参皂苷Rb1对大鼠脑缺血再灌注JAK2/STAT3信号转导通路的影响
Effect of ginsenoside Rb1 on the JAK2/STAT3 signal transduction pathway after cerebral ischemia-reperfusion in rats
摘要
Abstract
Objective To explore the effect of ginsenoside Rb1 on the JAK2/STAT3 signal transduction pathway after cerebral ischemia-reperfusion in rats for identifying the protection mechanism.Methods Made the model of middle cerebral artery occlusion/reperfusion(MCAO/R).Rats were randomly divided into sham group,vehicle group,high dosage group,and low dosage group of ginsenoside Rb1.The neurological deficit scores were assessed,the contents of TNF-α and IL-6 in ischemia brain tissue were detected by ELISA and the expressions of p-JAK2 and p-STAT3 were observated in the cortex by immunohistochemisty staining.Results (1) The neurological deficit scores were significantly decreased in ginsenoside Rb1 high dosage group compared with vehicle group(P＜0.05).(2) Ginsenoside Rb1 of high dosage significantly reduced the content of TNF-α and IL-6 in ischemia brain tissue (P＜0.05 or P＜0.01),the content of TNF-α was decreased significantly in low dosage group(P＜0.05),the content of IL-6 was also decreased but without statistical significance.(3) Both ginsenoside Rb1 of low and high dosage reduced the expressions of pJAK2 and p-STAT3 after cerebral ischemia-reperfusion(P＜0.05 or P＜0.01).Conclusion Ginsenoside Rb1 improved neurologic deficits' symptoms after cerebral ischemia-reperfusion,reduced the contents of TNF-α and IL-6 and inhibited the phosphorylation of JAK2 and STAT3.Therefore,Ginsenoside Rb1 could protect cerebral ischemia-reperfusion injury by regulating the JAK2/STAT3 signal transduction pathway.关键词
人参皂苷Rb1/脑缺血/再灌注损伤/JAK2/STAT3Key words
GRbl/brain ischemia/reperfusion injury/JAK2/STAT3引用本文复制引用
余录,胡光强,陈碧琼,涂华,杨朝鲜..人参皂苷Rb1对大鼠脑缺血再灌注JAK2/STAT3信号转导通路的影响[J].重庆医学,2013,42(17):1932-1934,3.基金项目
泸州医学院青年基金资助项目(201125). （201125）
