大豆科学2013，Vol.32Issue(3)：291-294,4.
吉林47大豆子叶节遗传转化体系的优化
Optimization on Cotyledonary-node Genetic Transformation System of Soybean Cultivar Jilin 47
摘要
Abstract
After infected by EHA105 caring plasmid pCAMBIA1301,the GUS staining of 10 soybean genotypes was carried out to detect the sensitivity for Agrobacterium-tumefaciens.Jilin 47,Jilin 35 and Suinong 10 were screened out as sensitive genenotypes.Jilin 47 was chosen as the genotype for optimization of soybean cotyledonary genetic transformation due to its good performance on shoot regeneration.Amending thiol compounds in liquid and solid co-cultivation medium could decrease the browning rate of explants and the best combination of 400 mg· L-1 L-Cys,248 mg· L-1 sodium thiosulfate and 300 mg· L-1 DTT were obtained through orthogonal test.The highest elongation rate was got when 1.0 mg· L-1 GA3 and 0.5 mg· L-1 IAA were added to shoot elongation medium.关键词
吉林47/子叶节/褐化/芽伸长Key words
Jilin 47 / Cotyledonary-node / Browning/ Shoot elongation分类
农业科技引用本文复制引用
孙昕,赵健如,闫帆,姬长媛,杨旭光,李景文,王英,王庆钰..吉林47大豆子叶节遗传转化体系的优化[J].大豆科学,2013,32(3):291-294,4.基金项目
转基因生物新品种培育重大专项(2011ZX08004-003) （2011ZX08004-003）
国家自然科学基金(30971808) （30971808）
"十二五"农村领域国家科技计划(2011BAD35B06-2) （2011BAD35B06-2）
吉林大豆产业技术体系. （）
