大豆科学2013，Vol.32Issue(3)：306-309,4.
大豆凝集素le2基因RNA干扰表达载体构建及转化的研究
Construction of RNAi Expressed Vector of Soybean Agglutinin le2 Gene and Transform Research
魏强 1李鲁华 1王春艳 1付永平 1马建 1曲静 1王丕武1
作者信息
- 1. 吉林农业大学农学院,吉林长春130118
- 折叠
摘要
Abstract
For the purpose of constructing RNAi expressed vector,and discussing the method to improve soybean quality,according to known conserved sequence(AY342212)of soybean agglutinin le2 gene in GenBank,right primer was designed and the conserved sequence was cloned.And the homology was 99.77％ contrast to the original sequence.Herein,using P3301-PF-NA-α'as the basic vector,the expression vector pCAMBIA3301-le2RNAi was constructed which contained RNA interference vector and bar gene through subcolone.The RNA interference expression vector was then transformed into cotyledon nodes of soybean Jinong 28 by Agrobacterium-mediated method.5 positive transgenic plants in T1 generation were confirmed by PCR detection and 23 seeds were harvested from T1 transgenic plants.The results provided a basis for soybean quality improvement.关键词
RNA干扰/大豆凝集素/载体构建/遗传转化Key words
RNAi / Soybean agglutinin/ Construction of vector/ Transform分类
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魏强,李鲁华,王春艳,付永平,马建,曲静,王丕武..大豆凝集素le2基因RNA干扰表达载体构建及转化的研究[J].大豆科学,2013,32(3):306-309,4.
