大豆科学2013，Vol.32Issue(3)：321-327,332,8.
保护性耕作对大豆生长发育及土壤微生物多样性影响
Effect of Conservation Tillage Systems on Soybean Growth and Soil Microbial Diversity
摘要
Abstract
The effect of conservation tillage on crop growth and soil microbial community play an important role in soil management.A long-term tillage experiment was conducted at Hailun Agro-Ecological Experiment Station of the Chinese Academy of Sciences.Tillage systems included no-tillage(NT),reduced tillage(RT) and rotary tillage (ROT).The study was to determine soybean growth and yield under conservation tillage,and analyze soil microbial diversity with Real-time QPCR and denaturing gradient gel electrophoresis(DGGE).NT and RT inhibited soybean growth and increased the disease severity of root rot at seedling stage.Soybean growth and yield was significantly increased under NT and RT compared with ROT at maturing stage.The highest yield of 2 507.3 kg·ha-1 was obtained under RT,which was increased by 3.9％ compared with ROT.The three tillage systems had no significant effect on the amount of bacteria,and the amount of fungi was NT ＞ RT ＞ ROT(P ＜0.05).The colony forming units (CFU)and the genomic DNA quality of soil fungi were increased by 31.9％ and 13.3％ under NT compared with ROT,respectively.The ratio of bacteria and fungi was increased significantly under RT and ROT.DGGE fingerprint showed that the soil bacterial diversity had no significant difference under three tillage systems,however,NT and RT increased soil fungal diversity.Principal component analysis(PCA) of DGGE banding patterns indicated that the bacterial community structure had a higher similarity under RT and ROT,and significant differences were observed on the fungal community structure under three tillage systems.关键词
保护性耕作/免耕/少耕/大豆/生长发育/微生物群落Key words
Conservation tillage / No-tillage / Reduced tillage / Soybean/ Growth / Microbial community分类
农业科技引用本文复制引用
向新华,魏巍,张兴义,许艳丽..保护性耕作对大豆生长发育及土壤微生物多样性影响[J].大豆科学,2013,32(3):321-327,332,8.基金项目
中国科学院知识创新工程(KZCX02-YW-408-3). （KZCX02-YW-408-3）
