大豆科学2013，Vol.32Issue(3)：333-335,3.
大豆组培苗水培生根与培养基生根比较研究
Tap Water Can be Used as Medium for Root Induction and Growth during Tissue Culture of Soybean
摘要
Abstract
Root-inducing is the final step for producing regenerated plantlets in the tissue culture of soybean [Glycine max (L.) Merr.].Here an easy,efficient root-inducing system for soybean shoots is reported,which is designated as tap-water rooting system,in which tap water is (without autoclaving)used to replace the traditional agar-containing medium for root induction and growth.Taking Zigongdongdou as material,the results demonstrated that the tap-water rooting system was good for shortening culture time,overcoming contamination of bacterium and fungus,increasing the rooting efficiency and decreasing the cost of tissue culture as exemplified by faster root inducing and elongating,and higher plantlet survive ratio of the tap-water system than that of agar-containing medium system.Although the amount of total roots from the tap-water system was less than that from agar plates,the plantlets from tap-water system could grow,flower and produce pods and seeds normally and no significant difference were found between the tap-water system and agar system.Hence,it is suggested that the easy rooting system should be used in the tissue culture of more soybean cultivar.关键词
大豆/水培生根/生根速度/成活率Key words
Soybean / Rooting in water culture / Growth rate / Survival rate分类
农业科技引用本文复制引用
胡凤,杨万年..大豆组培苗水培生根与培养基生根比较研究[J].大豆科学,2013,32(3):333-335,3.基金项目
国家自然科学基金(31270316). （31270316）
