大连工业大学学报2013，Vol.32Issue(3)：176-179,4.
响应面法优化黑曲霉固态发酵产木聚糖酶工艺
Optimization of xylanase production in Aspergillus niger solid fermentation by response surface methodology
周薇薇 1尹亚辉 1赵长新1
作者信息
- 1. 大连工业大学生物工程学院,辽宁大连 116034
- 折叠
摘要
Abstract
The xylanase production with Aspergillus niger FIP-09-24 in solid fermentation with wheat bran and barley as substance.The single factor experiments test and Plackett-Burman test suggested that carbon,nitrogen and water were the major factors on the synthesis of xylanase.Three-level response surface methodology based on the principle of central composite design was employed to analyze the significance and interactions of the above factors by mathematical modeling.The optimal conditions were determined by response surface analysis,which was the mass ratio of 3.8 ∶ 1 between wheat bran and barley residue,55.7％ of water content,2.0％ of nitrogen content,temperature 28 ℃,incubation time 60 h.The enzyme activity of the fermentation culture can reach up to 66 002 U/g.关键词
黑曲霉/木聚糖酶/固态发酵/响应面法Key words
Aspergillus niger / xylanase/ solid-state fermentation/ response surface methodology分类
轻工纺织引用本文复制引用
周薇薇,尹亚辉,赵长新..响应面法优化黑曲霉固态发酵产木聚糖酶工艺[J].大连工业大学学报,2013,32(3):176-179,4.
