南方医科大学学报2013，Vol.33Issue(6)：798-803,6.DOI:10.3969/j.issn.1673-4254.2013.06.04
趋化因子CXCL14在骨肉瘤中的表达及其与预后的关系
Expression of chemokine CXCL14 in primary osteosarcoma and its association with prognosis
摘要
Abstract
Objective To detect the expression of CXCL14 in human osteosarcoma cell lines and tissues and investigate its association with the prognosis of the patients.Methods RT-PCR,enzyme-linked immunosorbnent assay (ELISA) and real-time PCR were used to detect the expression of CXCL14 in 4 osteosarcoma cell lines and in 40 pairs of osteosarcoma tissues and adjacent muscular tissues.CCK8 assay and colony formation assay was used to assess the effect of CXCL14 suppression mediated by two specific siRNAs on the proliferation of U2OS osteosarcoma cells.Immunohistochemistry was performed to detect the expression of CXCL14 in 58 osteosarcoma tissues,and Kaplan-Meier method and log-rank test were performed for survival analysis of the patients.Results Significant up-regulation of CXCL14 expression was found in the osteosarcoma cell lines and in osteosarcoma tissues compared with the adjacent muscles (P＜0.01).In U2OS cell,suppression of CXCL14 expression by siRNA significantly inhibited the cell proliferation (P＜0.01) and colony formation rate (P＜0.05).Kaplan-Meier survival analysis indicated that patients with high CXCL14 expression had worse prognosis than those with low CXCL14 expression (P=0.02).Conclusion CXCL14 is up-regulated in both osteosarcoma cell lines and primary osteosarcoma tissues to promote the proliferation of osteosarcoma cells.A high CXCL14 expression in osteosarcoma tissues is associated with a poor prognosis,suggesting the that CXCL14 serve as a potential therapeutic target for osteosarcoma.关键词
CXCL14/骨肉瘤/趋化因子Key words
CXCL14/ osteosarcoma/ chenokine引用本文复制引用
卢金昌,王晋,雍碧城,宋国徽,赵志强,唐清连,邹昌业,尹军强,谢显彪..趋化因子CXCL14在骨肉瘤中的表达及其与预后的关系[J].南方医科大学学报,2013,33(6):798-803,6.基金项目
国家自然科学基金(81272940,81072193) （81272940,81072193）
Supported by National Natural Science Foundation of China (81272940,81072193). （81272940,81072193）
