南方医科大学学报2013，Vol.33Issue(6)：812-818,7.DOI:10.3969/j.issn.1673-4254.2013.06.06
Id1/Id3在子宫内膜癌中的表达及对子宫内膜癌细胞生物学的作用
Expression of Id1 and Id3 in endometrial carcinoma and their roles in regulating biological behaviors of endometrial carcinoma cells in vitro
摘要
Abstract
Objective To investigate the expression of irhibitor of DNA differentiation/DNA binding 1 (Id1) and Id3 in endometrial carcinoma and explore their roles in regulating the proliferation,invasion,migration and adhesion of endometrial carcinoma cells in vitro.Methods Id1 and Id3 expression in 4 fresh endometrial cancer tissue specimens and matched adjacent tissues were detected using Western blotting.Two endometrial cancer cell lines,HEC-1-B and RL-952,were both divided into 4 groups,namely the untreated group,blank virus group,promoter group and Id1/Id3 double-knockdown group,and their expressions of MMP2,CXCR4 and P21 were detected by qRT-PCR and Western blotting.The proliferation,invasion,migration and adhesion of the cells were evaluated with MTT,Transwell,wound-healing,and adhesion assays.Results Endometrial carcinoma tissues showed significantly higher Id1 and Id3 expression than the adjacent tissues (P＜0.05).In the two endornetrial carcinoma cell lines,Id1/Id3 double-knockdown significantly decreased MMP2 and CXCR4 expression and increased P21 expression at both mRNA and protein levels (P＜0.05),and resulted in suppressed cell proliferation,invasion,migration and adhesion.Conclusions Id1 and Id3 expressions are up-regulated in endometrial carcinoma to promote the proliferation,invasion,migration and adhesion of the tumor cells by increasing MMP2 and CXCR4 expression and reducing P21 expression.Therapies targeting Id1/Id3 can be a novel strategy for treatment of endometrial carcinoma.关键词
Id1/Id3/子宫内膜癌/RNA干扰/腺病毒/DNA分化Key words
Id1/ Id3/ endometrial carcinoma/ RNA interference/ adenovirus/ DNA differentiation引用本文复制引用
孙丽丽,李学农,刘国炳..Id1/Id3在子宫内膜癌中的表达及对子宫内膜癌细胞生物学的作用[J].南方医科大学学报,2013,33(6):812-818,7.基金项目
广东省自然科学资金(S2012010009351) （S2012010009351）
广东省科技计划项目(2010B031600091) （2010B031600091）
