南方医科大学学报2013，Vol.33Issue(6)：838-841,4.DOI:10.3969/j.issn.1673-4254.2013.06.10
毒蕈碱乙酰胆碱M2/M4受体亚型在调节脊髓背角神经元谷氨酸能递质释放中的作用
Role of muscarinic cholinergic receptor subtypes in regulating glutamatergic synaptic transmission in rat spinal dorsal horn
摘要
Abstract
Objective To investigate the role of muscarinic cholinergic receptor (mAChR) subtypes in the regulation of glutamatergic input to the spinal dorsal horn neurons and the possible mechanism.Methods Whole-cell voltage-clamp recordings on acute spinal slice was utilized to investigate the effect of activation of mAChRs and blockade of M2/M4 subtypes on glutamatergic synaptic transmission in rat spinal dorsal horn neurons.Results The nonselective mAChRs agonist oxotremorine-M concentration-dependently decreased the amplitude of monosynaptic and polysynaptic evoked glutamate-mediated excitatory postsynaptic currents (eEPSCs) in most of the neurons.The M2/M4 antagonist himbacine completely blocked the inhibitory effect of oxotremorine-M in 92.3％ of monosynaptic and 75％ of polysynaptic neurons in the spinal cord slices.In the remaining 16％ neurons,himbacine partially blocked the inhibitory effect of oxotremorine-M.Conclusions Activation of mAChRs in the spinal cord attenuates synaptic glutamate release to the dorsal horn neurons mainly through M2 and M4 receptor subtypes,indicating that a presynaptic inhibition in the spinal cord may be involved in the regulation of nociception by the cholinergic system and mAChRs.关键词
毒蕈碱胆碱能受体/脊髓背角/突触前抑制/谷氨酸能突触传递/兴奋性突触后电流Key words
muscarinic cholinergic receptors/ spinal dorsal horn/ presynaptic inhibition/ glutamic synaptic transmission/evoked glutamate-mediated excitatory postsynaptic currents引用本文复制引用
杜威,郭英,袁维秀..毒蕈碱乙酰胆碱M2/M4受体亚型在调节脊髓背角神经元谷氨酸能递质释放中的作用[J].南方医科大学学报,2013,33(6):838-841,4.基金项目
教育部留学回国人员科研启动基金(教外司留[2011]1568) （教外司留[2011]1568）
