南方医科大学学报2013，Vol.33Issue(6)：930-933,4.DOI:10.3969/j.issn.1673-4254.2013.06.32
Exendin-4对肿瘤坏死因子-α诱导的单核细胞趋化蛋白-1表达的影响
Effect of exendin-4 on monocyte chemoattractant protein-1 expression in cultured rat glomerular mesangial cells induced by tumor necrosis factor-α in vitro
摘要
Abstract
Objective To investigate the effect of exendin-4 on the expression of monocyte chemoattractant protein-1 (MCP-1)and fibronectin (FN) in rat glomerular mesangial cells in vitro.Methods Rat glomerular mesangial cells were divided into 5 groups,namely control group,tumor necrosis factor-α (TNF-α) group (10 ng/ml),TNF-α (10 ng/ml)+E1 (1 nmol/L exendin-4)group,TNF-α (10 ng/ml)+ E5 (5 nmol/L exendin-4) group,and TNF-α (10 ng/ml)+ E10 (10 nmol/L exendin-4) group.After cultured 24 h or 48 h,RNA were extracted to determine the expression of MCP-1 with real-time PCR,the supematant were collected to determine the expression of MCP-1 and FN with ELISA.Results Compared with control group,the cells treated with TNF-α for 24 h showed significantly increase the expression of MCP-1 and FN (P＜0.01),exendin-4 significantly reduced the expression of MCP-1 and FN in TNF-α+E5 group and TNF-α+E10 group (P＜0.05).After 48h incubation,the expression of MCP-1 and FN increased significantly in TNF-α group (P＜0.01),which was lowered by exendin-4 in TNF-α+E1,TNF-α+E5 and TNF-α+E10 groups (P＜0.05).Conclusion Exendin-4 has an intrinsic capability to concentration-and time-dependently inhibit TNF-α-induced expression of MCP-1 and FN in rat mesangial cells,suggesting the beneficial effect of exendin-4 in preventing and treating diabetic nephropathy.关键词
肿瘤坏死因子-α/单核细胞趋化蛋白-1/纤维连接蛋白/糖尿病肾病/exendin-4Key words
tumor necrosis factor-α/ monocyte chemoattractant protein-1/ fibronectin/ diabetic nephropathy/ exendin-4引用本文复制引用
江颖娟,薛耀明,张倩,张艳飞,袁园..Exendin-4对肿瘤坏死因子-α诱导的单核细胞趋化蛋白-1表达的影响[J].南方医科大学学报,2013,33(6):930-933,4.基金项目
广东省科技计划项目(2009B080701020) （2009B080701020）
