福建农业学报2013，Vol.28Issue(4)：357-360,4.
金线莲白绢病病原菌分离及分子鉴定
Isolation and Molecular Identification of Southern Blight Pathogen in Anoectochilus roxburghii (Wall.) Lindl.
摘要
Abstract
In this study,the suspected Pathogen of Anoectochilus roxburghii Southern blight was isolated by tissue separation and purified from samples with typical symptoms.Then the pathogen was identified by pathogenicity test,pathogen morphology and molecular biology methods.The results showed that the strain from southern blight infected Anoectochilus tissues collected from Fujian were isolated.Mycelium was white,velvet-like,close to the culture medium,radially extended to the surrounding containment and branches,colonies formed on the surface of sclerotia,sclerotia early white,then change to yellow,and finally was dark brown,spherical or irregular spherical,smooth and shiny surface.The isolated strain has pathogenicity to tested Anoectochilus.The pathogen was identified pathogenically,morphologically and molecularly.Based on these data,it can be confirmed that the pathogen was Sclerotium rolfsii Sacc.关键词
金线莲/白绢病/病原菌/鉴定Key words
Anoectochilus roxburghii (Wall.) Lindl./ southern blight/ pathogens/ identification分类
农业科技引用本文复制引用
赵云青,黄颖桢,陈菁瑛,刘保财..金线莲白绢病病原菌分离及分子鉴定[J].福建农业学报,2013,28(4):357-360,4.基金项目
农业部公益性行业(农业)科研专项(201303117) （农业）
福建省农业科学院青年科技人才创新基金(2011QA-6) （2011QA-6）
福建省种业创新与产业化工程(2011FJZY) （2011FJZY）
