福建农业学报2013，Vol.28Issue(5)：413-418,6.
半滑舌鳎(Cynoglossus Semilaevis)EB1基因的克隆、表达及其对细胞迁移能力的相关性分析
Cloning, Expression Analysis and Cell Migration Analysis of EB1 in Cynoglossus Semilaevis
摘要
Abstract
EB1,as a microtubule plus end binding protein,plays an important role in the microtubule polymerization and microtubules equipment found on the protein complex.In current experiment,EB1 gene was cloned on the basis of half smooth tongue sole cDNA library.The gene contained an open reading frame of 777 bp code that encoded putative 258 amino acids with a calculated molecular mass of 29.497 kDa.The deduced amino acid sequence was aligned with other species,and they had the high identity and homology.It was found that the proteins included two conservative domain structure,CH and EB1,and two conservative trigeminy amino acid sites,FYF and EEF/Y.Homologous clustering analysis results showed that the EB1 proteins could well reflect the species development of evolution from lower to higher states.Real-time fluorescent quantitative PCR results showed that the genes expressed in all the tissues selected and,the highest level existed in ovary,which was higher 14.6 times than the lowest amount of liver tissue.Consistent with qRT-PCR results,cell mobility showed consistency with the expression of EB1 in tissues.关键词
半滑舌鳎/EB1/微管末端/细胞迁移Key words
Cynoglossus Semilaevis/EB1/microtubule plus end/cell migration分类
农业科技引用本文复制引用
刘聪辉,高金宁,辛念,马丽曼,侯熠,齐洁..半滑舌鳎(Cynoglossus Semilaevis)EB1基因的克隆、表达及其对细胞迁移能力的相关性分析[J].福建农业学报,2013,28(5):413-418,6.基金项目
国家自然科学基金项目(31072204) （31072204）
