福建农业学报2013，Vol.28Issue(5)：463-467,5.
中国水仙叶绿素a/b结合蛋白基因Ntcab1的克隆与序列分析
Cloning and Sequence Analysis on Gene of Chlorophyll a/b-binding Protein from Narcissus tazetta var.chinensis
摘要
Abstract
From a cDNA fragment of the suppression subtractive hybridization of Narcissus tazetta var.chinensis induced by paclobutrazol,the gene of the light-harvesting chlorophyll a/b-binding protein,Ntcab1,was isolated and cloned by RACE and RT-PCR.The full length of Ntcab1 cDNA was determined to be 1 012 bp with a 798 bp ORF,which encoded a protein of 265 amino acids with a predicted molecular weight of 28.155 kD and a theoretical isoelectric point of 5.48.The phylogenetic analysis showed that Ntcab1 could be clustered together with LHCb1.1 from Arabidopsis thaliana,and had a close relationship with those in Ophiopogon japonicus (ACD64978.1) and Lycoris radiata (ADC35579.1).The semi-quantitative RT-PCR revealed that the expression of Ntcab1 was higher during sprouting and early flowering after it was sprayed with paclobutrazol than water.关键词
中国水仙/叶绿素a/b结合蛋白/基因克隆Key words
Narcissus tazetta var.Chinensis/light-harvesting chlorophyll a/b-binding protein/gene cloning分类
农业科技引用本文复制引用
林江波,王伟英,邹晖,戴艺民..中国水仙叶绿素a/b结合蛋白基因Ntcab1的克隆与序列分析[J].福建农业学报,2013,28(5):463-467,5.基金项目
福建省自然基金项目(2010J01090) （2010J01090）
福建省农业科学院农业科技创新重点领域项目(2007YCXZ02) （2007YCXZ02）
