分析测试学报2013，Vol.32Issue(6)：699-704,6.DOI:10.3969/j.issn.1004-4957.2013.06.008
高效液相色谱-串联质谱法同时测定保健品中非法添加的六种减肥化学品
Simultaneous Determination of Six Chemical Drugs Illegally Added in Dietary Supplement by High Performance Liquid Chromatography Tandem Mass Spectrometry
摘要
Abstract
A high performance liquid chromatography-mass spectrometric (HPLC-MS/MS) method was proposed for the determination of 6 anti-obesity chemical drugs (sibutramine,N-di-desmethyl sibutramine,fenfluramine,phenolphthalein,rimonabant,orlistat) added illegally into health foods.The conditions of extraction procedures and the parameters of HPLC-MS/MS were optimized.The drugs were extracted with methanol,and separated on a C8 chromatographic column using a mixture of acetonitrile and 10 mmol/L ammonium acetate solution as mobile phase with gradient elution.The detection of 6 targeted compounds was performed by positive-ion electrospray ionization mass spectrometry(ESI MS) under multiple reaction monitoring (MRM) mode.Good linearities for 6 compounds were obtained in the range of 0.001-0.5 mg/L with correlation coefficients greater than 0.999.The spiked recoveries were in the range of 81％-100％ with RSDs of 4.8％-6.9％.The detection limits of these drugs were up to 0.5 μg · L-1.The results indicated that this method was simple,rapid and accurate,and was successfully applied in the examination of examine chemical drugs illegally added in the dietary supplement.关键词
减肥药/液相色谱-串联质谱/保健品Key words
weight-loss chemical drugs/ high performance liquid chromatography-tandem mass spectrum(HPLC-MS/MS) / functional foods分类
化学化工引用本文复制引用
黄芳,吴惠勤,黄晓兰,邓欣,罗辉泰,朱志鑫,林晓珊..高效液相色谱-串联质谱法同时测定保健品中非法添加的六种减肥化学品[J].分析测试学报,2013,32(6):699-704,6.基金项目
广东省科技计划项目(2012B040302007) （2012B040302007）
