分析化学2013，Vol.41Issue(7)：973-978,6.DOI:10.3724/SP.J.1096.2013.21045
基于β-环糊精主客体识别作用的均相DNA杂交电化学生物分子识别研究
Electrochemical Biomolecule Recognition Through DNA Homogenous Hybridization Based on Host-guest Recognition Technology
摘要
Abstract
A sensitive electrochemical DNA biosensing through homogenous hybridization was fabricated based on host-guest recognition system due to the very strong inclusion between β-cyclodextrin (β-CD) and m-toluic acid (mTA).fβ-CD,as the host molecule,was electropolymerized on the poly (N-acetylaniline)-modified glassy carbon electrode,and mTA,as the guest,was labeled on the probe DNA using acid and amine condensation reaction.Using the host-guest recognition,the single probe DNA and hybrid double-stranded DNA were connected to the electrode surface.Two intercalators were used as electrochemical indicators to detect DNA hybridization,methylene blue (MB) and daunorubicin (DNM).The electrochemical signals of MB were linear with the concentrations of complementary DNA from 2.0× 10-12 mol/L to 2.0× 10-10 mol/L with a detection limit of 7.6× 10-13 mol/L.The electrochemical responses of DNM were linear with the concentrations of complementary from 1×10-12 mol/L to 1 ×10-9 mol/L with a detection limit of 6.0×10-13 mol/L.This DNA biosensing technology exhibited excellent reproducibility and stability.关键词
β-环糊精/主客体识别/DNA均相杂交/电化学检测Key words
β-Cyclodextrin / Host-guest recognition/ Homogenous hybridization / Electrochemistric detection引用本文复制引用
陈琛,常竹,杨丽珠,张帆,何品刚,方禹之..基于β-环糊精主客体识别作用的均相DNA杂交电化学生物分子识别研究[J].分析化学,2013,41(7):973-978,6.基金项目
本文系国家自然科学基金项目(No.21275054)资助 （No.21275054）
