法医学杂志2013，Vol.29Issue(3)：164-167,4.DOI:10.3969/j.issn.1004-5619.2013.03.002
Spry1和MAPK蛋白在病毒性心肌炎心肌组织中的表达
Myocardial Expression of Spry1 and MAPK Proteins of Viral Myocarditis
摘要
Abstract
Objective To discuss the myocardial expression of Spry1 and MAPK proteins of viral myocarditis (VMC),to reveal its mechanism of sudden death,and to provide guides for forensic identification of sudden cardiac death.Methods Thirty Balb/c male mice were randomly divided into VMC group and control group,inoculated intraperitoneally with Coxsackievirus B3 and Eagel's solution,respectively.After the mice were sacrificed,the cardiac tissues of the mice were taken to proceed regular pathological examination.The changes of Spryl protein,Spryl mRNA and MAPK protein were detected by immunohistochemistry,Westem blotting and real-time PCR.Results Under light microscope,the pathologic changes included myocardial interstitial edema,inflammatory cells infiltration,myocardial necrosis,and focal and patchy necrosis of myocardial fiber in VMC group.The expression of Spryl protein in VMC group was lower than that in control group (P＜0.05).There was slightly decreased expression of Spryl of the mRNA level in VMC group (P＞0.05).But the MAPK protein expression in VMC group was higher than that in control group (P＜0.05).Conclusion The pathway of MAPK/ERK involving Spry1 protein accelerates the expression of collagen,which may contribute to arrhythmia,heart failure and even sudden cardiac death.关键词
法医病理学/猝死,心脏/病毒性心肌炎/Spry蛋白/MAPK蛋白Key words
forensic pathology/ death, sudden, cardiac/ vital myocarditis/ Spry protein/ MAPK protein分类
社会科学引用本文复制引用
许弘飞,孟林,姚健,谷振勇,刘国庆,沈忆文,赵子琴..Spry1和MAPK蛋白在病毒性心肌炎心肌组织中的表达[J].法医学杂志,2013,29(3):164-167,4.基金项目
国家自然科学基金资助项目(81172897) （81172897）
南通市自然科学基金资助项目(BK2011053) （BK2011053）
