法医学杂志2013，Vol.29Issue(3)：202-205,208,5.DOI:10.3969/j.issn.1004-5619.2013.03.012
山东地区汉族人群15个X-STR基因座的遗传多态性
Genetic Polymorphisms of 15 X-STR Loci in Shandong Han Population
摘要
Abstract
Objective To investigate the genetic polymorphisms of 15 X-STR loci in Shandong Han population in order to establish the forensic application database.Methods The multi-PCR primers of these loci were designed by Primer Premier 5.0 software and labeled by 4 fluoresceins (FAM,VIC,NED and TET).The developed multi-PCR was used to investigate 15 X-STR loci (DXS10011,DXS101,GATA165B12,DXS6795,DXS6800,DXS6801,DXS6803,DXS7132,DXS7133,DXS7423,DXS7424,DXS8377,DXS8378,DXS9898 and HPRTB) selected from the X chromosome of 481 unrelated individuals (295 females and 186 males) in Shandong Han population.Results Among the 15 X-STR loci,GATA165B12,DXS6800,DXS6803,DXS7133 and DXS7423 showed moderate polymorphisms,while the rest 10 X-STR loci showed high polymorphisms (PIC＞0.5 and H＞0.5).No shared haplotype was detected among the males in Shandong Han population.Conclusion The developed multi-PCR system with fluorescence detection provides an effective way to establish X-STR loci database of population genetics in Shandong Han population and shows its forensic application.关键词
法医遗传学/多态现象,遗传/X-STR/山东/汉族Key words
forensic genetics/ polymorphism, genetic/ X-STR/ Shandong/ Han nationality分类
社会科学引用本文复制引用
马腾,徐婧,杨亚军,孙宽,薛付忠,金力,李士林..山东地区汉族人群15个X-STR基因座的遗传多态性[J].法医学杂志,2013,29(3):202-205,208,5.基金项目
国家高技术研究发展计划(863计划)(SS2012AA021303) （863计划）
国家自然科学基金面上项目(31071096) （31071096）
