福州大学学报（自然科学版）2013，Vol.41Issue(3)：397-402,6.DOI:10.7631/issn.1000-2243.2013.03.0397
一株产高温纤维素酶真菌的筛选、鉴定及酶学性质研究
Isolation and identification of hot-resisitant cellulase fungus and study on enzymic properties
摘要
Abstract
A wild strain with high temperature tolerance cellulase was isolated from the soil samples near the paper mill.The strain was identified and named as Aspergillus terreus XM5 by its morphological analysis and ITS rDNA sequence.The pattern of cellulase synthesis was associated with the growth of Aspergillus terreus XM5 in submerged fermentation with rice straw as the sole carbon source.The optimum temperature for the cellulase activity was at 65 ℃ and the cellulase maintained more than 40％ of its maximum activity after incubation for 2 h at 80 ℃.The optimum pH for the cellulase activity was at 4.0; and the eellulase retained stability in the pH range of 4.0 ～ 7.0 after incubation for 3 h.The results showed that the cellulase from the strain has high pH and temperature stability.关键词
纤维素酶/土曲霉/筛选鉴定/酶学性质Key words
cellulose / Aspergillus terreus / isolation identification / enzyme propeirties分类
生物科学引用本文复制引用
严芬,李源涛,李丽莎,林娟..一株产高温纤维素酶真菌的筛选、鉴定及酶学性质研究[J].福州大学学报（自然科学版）,2013,41(3):397-402,6.基金项目
福建省科技厅科研资助项目(2010J05073) （2010J05073）
福州大学人才基金资助项目(XRC-0946) （XRC-0946）
福建省大学生创新创业训练计划资助项目(FJ1210386022) （FJ1210386022）
