广东医学2013，Vol.34Issue(11)：1637-1640,4.
川芎嗪对角膜基质细胞中趋化因子受体CXCR4的影响
Effects of tetramethylpyrazine on chemokine receptor CXCR4 of rat corneal stromal cells
摘要
Abstract
Objective To investigate the effects of tetramethylpyrazine ( TMP) on rat corneal stromal cells and the correlation between them. Methods Rat corneal stromal cells from 8 to 10 weeks SD rats were isolated and cultured . CXCR4 expression was measured with cell immunofluorescence method . Comeal stromal cells were treated with TMP for 48 h. Methylthiazolyl tetrazolium (MTT) assay was performed to assess cell proliferation , while reverse transcription PCR (RT -PCR) and Western blot were adopted to evaluate CXCR4 expression. Results The viability and growth of rat cor-neal stromal cells were significantly inhibited with ( P < 0. 05) ; so was the mRNA and protein expression of CXCR 4 ( P < 0. 05). Moreover, TMP significantly down - regulated the expression of CXCR4 in rat corneal stromal cells in a dose - dependent manner. Conclusion TMP remarkably inhibits the viability and growth of rat corneal stromal cells by down -regulating chemokine receptor CXCR4.关键词
大鼠角膜基质细胞/CXCR4/川芎嗪/纤维化Key words
rat comeal stromal cells / CXCR4/ tetramethylpyrazine / fibrosis引用本文复制引用
田思佳,杨颖,余克明,Ming Wang,庄菁..川芎嗪对角膜基质细胞中趋化因子受体CXCR4的影响[J].广东医学,2013,34(11):1637-1640,4.基金项目
国家自然科学基金面上项目(编号:30872811) （编号:30872811）
