国际生殖健康/计划生育杂志2013，Vol.32Issue(2)：87-90,110,5.
Hsa miR-7慢病毒表达载体的构建及其在人卵巢癌细胞HO8910-PM中表达的有效性
Construction of the Hsa miR-7 Lentiviral Expression Vector and its Expression in Human Ovarian Cancer Cell Line HO8910-PM
摘要
Abstract
Objective:To construct the Hsa miR-7 lentiviral vector,and to detect its effectiveness in human ovarian cancer cell line HO8910-PM.It is the bases of functional study of Hsa miR-7 in ovarian cancer.Methods:Hsa miR-7 was amplified from the genomic DNA and inserted into pLVX-IRES-ZsGreen1 vector after double digestion to generate pLVX-miR7-IRES-ZsGreen1 vector.The vector was then confirmed by PCR and DNA sequencing.HO8910-PM was infected by the concentrated lentivirus 293T produced.Pre-miR-7,miR-7 and gene EGFR were assessed by qPCR.Results:The recombinant lentiviral vector was successfully established and confirmed by PCR and DNA sequencing.Pre-miR-7 (t=17.909,P=0.004) was integrated into the genome of HO8910-PM and miR-7 (t=35.320,P=0.024) expression was enhanced effectively by qPCR.Moreover,miR-7 target gene EGFR was also reduced both in mRNA (t=8.83,P=0.005) and protein (t=22.14,P=0.002)levels after miR-7 overexpression in HO8910-PM.Conclusions:The Hsa miR-7 lentiviral expression vector was successfully constructed,and the Hsa miR-7 overexpressed in HO8910-PM cell line.关键词
微小RNAs/慢病毒属/卵巢肿瘤/受体,血管内皮生长因子Key words
MicroRNAs/ Lentivirus/ Ovarian neoplasms/ Receptors, vascular endothelial growth factor引用本文复制引用
王文文,邱丽华..Hsa miR-7慢病毒表达载体的构建及其在人卵巢癌细胞HO8910-PM中表达的有效性[J].国际生殖健康/计划生育杂志,2013,32(2):87-90,110,5.基金项目
国家自然基金委面上项目(81072138) （81072138）
