南方农业学报2013，Vol.44Issue(5)：717-721,5.DOI:10.3969/j:issn.2095-1191.2013.5.717
红腺忍冬组织培养快繁技术
Tissue culture and rapid propagation of L.hypoglauca
摘要
Abstract
[Objective]The tissue culture of L.hypoglauca for dominant Lonicera confusa DC.cultivars in Guangxi was studied to provide technical support for the industrialized production of L.hypoglauca seedlings in Guangxi.[Method] L.hypoglauca axillary buds sterilized by 0.1％ HgCl2(Mercuric chloride) were used as explants for tissue culture,Different hormone combinations (6-BA,NAA and MET)were added in MS medium to conduct buds induction,multiplication and rooting induction,etc.[Result]Comparing all the sterilization treatments,the most suitable one was that dipping shoots in 0.1％ HgCl2 solution with tween-80 for 7 min,and the contamination rate was 16.0％.The explants sampled in spring had the lowest pollution rate and best axillary bud induction,while the winter samples did the worst.As a result,more suitable culture medium for buds induction was MS+6-BA 2.0 mg/L,and multiplication culture of axillary buds was found as MS +6-BA 2.0 mg/L+NAA 0.1 mg/L.MS medium with NAA 0.1 mg/L and MET 3.0 mg/L was much more effective; widely used in L.hypoglauca industrialized breedings,and large-scale cultivation in Guangxi.关键词
山银花/红腺忍冬/组织培养/快繁Key words
L.confusa DC/ L.hypoglauca/ tissue culture/ rapid propagation分类
农业科技引用本文复制引用
农艳丰,杨美纯,苏小茴,岑湘涛..红腺忍冬组织培养快繁技术[J].南方农业学报,2013,44(5):717-721,5.基金项目
National Nstural Science Foundation of China(31160300) （31160300）
