南方农业学报2013，Vol.44Issue(5)：722-729,8.DOI:10.3969/j:issn.2095-1191.2013.5.722
甘蔗乙烯受体Sc-ERS启动子的克隆与序列分析
Cloning and sequence analysis of promoter in sugarcane ethylene receptor gene (Sc-ERS)
摘要
Abstract
[Objective]The present study was conducted to clone the promoter sequence of sugarcane ethylene receptor (Sc-ERS) and analyze its acting elements.[Method]Sc-ERS promoter sequence was cloned from the genomic DNA of sugarcane cultivar ROC22 with chromosome walking technology based on thermal asymmetric interlaced PCR principle,and the acting elements of Sc-ERS were analyzed with PlantCARE and PLACE online tools.[Result]The gene promoter sequence of Sc-ERS was cloned,and it was 1410 bp in size sharing 82％ and 80％ homology with the sequences of ERS25 and ERS14 of corn,respectively.PlantCARE online analysis results showed that the gene promoter sequence of Sc-ERS had promoter acting elements TATA-BOX and CAAT-BOX,light-responsive elements,drought-induced MYB binding sites,methyl jasmonateresponsive element,salicylic acid response element,endosperm expression cis-regulatory elements,heat stress responsive elements and so on.[Conclusion]A 1410 bp promoter sequence of Sc-ERS was cloned from sugarcane genomic DNA,which contained several specific acting elements,indicating that its expression could be regulated by circadian rhythm,hormone,drought,light and other factors.关键词
甘蔗/乙烯受体基因/启动子序列/克隆Key words
sugarcane/ ethylene receptor gene / promoter sequence/ clone分类
农业科技引用本文复制引用
黄静丽,牛俊奇,朱惠,杨丽涛,李杨瑞,王爱勤..甘蔗乙烯受体Sc-ERS启动子的克隆与序列分析[J].南方农业学报,2013,44(5):722-729,8.基金项目
国家"863"计划项目(2013AA102604) （2013AA102604）
国际科技合作项目(2009DFA30820,2013DFA31600) （2009DFA30820,2013DFA31600）
广西自然科学基金项目(2011GXNSFA018069,2011GXNSFF018002) （2011GXNSFA018069,2011GXNSFF018002）
广西自治区主席科技资金项目(11166-02) （11166-02）
