南方农业学报2013，Vol.44Issue(5)：730-734,5.DOI:10.3969/j:issn.2095-1191.2013.5.730
薄壳山核桃MADS-box基因保守片段的克隆与序列分析
Cloning and sequence analysis of MADS-box gene conservative fragment from pecan (Carya illinoensis)
摘要
Abstract
[Objective]The conservative fragment sequence of MADS-box genes from pecan was cloned and their phylogeny was assayed in order to provide references for cloning pecan new MADS-box family genes related to flower development and exploring molecular mechanism of pecan flower development.[Method]The total RNA was extracted from male flower of pecan variety 'Mahan' and cDNA was synthesized using inverse transcription.The conservative fragment of MADS-box genes was cloned using RT-PCR,and the phylogenetic analysis was conducted using the deduced amino acid sequences with known Arabidopsis MADS-box gene subfamily.[Result]Twenty-eight cDNA fragments of MADS-box gene were obtained by RT-PCR,and the length of these fragments was 137 bp including the initiation codon of genes.The homology of these nucleotide sequences was between 65.7％ and 98.5％.The 11 deduced amino acid sequences were different.According to phylogenetic tree analysis,all these gene fragments involving different types of gene in ABCDE model were divided into the Arabidopsis MADS-box gene subfamily.[Conclusion]There were many MADS-box genes in pecan.These gene fragments included all types of floral development genes in ABCDE model.关键词
薄壳山核桃/MADS-box基因/RT-PCR/克隆/序列分析Key words
pecan/ MADS-box gene/ RT-PCR/ sequence analysis分类
农业科技引用本文复制引用
莫正海,张计育,翟敏,贾晓东,宣继萍,李永荣,郭忠仁..薄壳山核桃MADS-box基因保守片段的克隆与序列分析[J].南方农业学报,2013,44(5):730-734,5.基金项目
国家自然科学基金项目(31200502) （31200502）
江苏省科技支撑计划项目(BE2012338) （BE2012338）
江苏省农业科技自主创新资金项目[CX(12)2012] （12）
南京市科技计划项目(201101025) （201101025）
