南方农业学报2013，Vol.44Issue(5)：751-754,4.DOI:10.3969/j:issn.2095-1191.2013.5.751
红茄子叶的不定芽诱导及植株再生
Adventitious bud induction of S.integrifolium cotyledon and plantlet regeneration
摘要
Abstract
[Objective]The present experiment was performed to study the effects of different hormone concentrations and combinations on induction of adventitious bud and root with the aim of establishing a highly efficient regeneration system of S.integrifolium to provide references for the research of eggplant genetic transformation.[Method]With the cotyledon of S.integrifolium as explants,the different hormone combinations (0.5-2.0 mg/L 6-BA,0.1-0.5 mg/L IAA and 0-0.5 mg/L IAA)were added in MS medium to induce the adventitious bud and root,respectively.Subsequently the optimal differential medium and rooting medium were screened.[Result]The germination rate of adventitious buds increased with the increase in 6-BA concentration from 0.5 mg/L to 2.0 mg/L in MS medium.The germination rate of adventitious buds increased initially with IAA concentration increasing from 0.1 mg/L to 0.5 mg/L in MS medium,followed by a decrease.However,2.0 mg/L 6-BA plus 0.3 mg/L IAA showed ideal induction effects on adventitious buds with 86％ germination rate.As the IAA concentration in MS medium increased,the rooting rate decreased at first,then increased.The highest germination rate reached 100％.[Conclusion]The best medium for initiation of adventitious bud of cotyledon was MS+2.0 mg/L 6-BA + 0.3 mg/L IAA,and the optimal rooting medium was MS+0.1 mg/L IAA.关键词
红茄/子叶/不定芽诱导/植株再生/培养基筛选Key words
S.integrifolium/cotyledon/induction of adventitious bud/plantlet regeneration分类
农业科技引用本文复制引用
方岩岩,王益奎,王红,李文嘉,黎炎..红茄子叶的不定芽诱导及植株再生[J].南方农业学报,2013,44(5):751-754,4.基金项目
广西自然科学基金项目(2011GXNSFB018025) （2011GXNSFB018025）
广西农业科学院基本科研业务专项项目[200816(基)] （基）
广西农业科学院科技发展基金项目(200904Z,桂农科2011JM15) （200904Z,桂农科2011JM15）
