南方农业学报2013，Vol.44Issue(5)：810-814,5.DOI:10.3969/j:issn.2095-1191.2013.5.810
杉木茎段不定芽诱导及植株再生条件筛选
Screening conditions of adventitious bud induction and plantlet regeneration for Cunninghamia lanceolata
摘要
Abstract
[Objective]Conditions of the adventitious bud induction and plant regeneration for Cunninghamia lanceolata were screened and rapid propagation techniques for Chinese fir in Guangxi was explored to provide references for supply and tissue culture of Chinese fir seedlings.[Method]Using base branches of superior Chinese fir species as testing materials,stem explant disinfection,adventitious bud induction and plant regeneration were the focal points in the current research.[Result] (1)The best disinfection method was treating stem explants with 75％ ethanol for 30 s and 0.1％ mercuric chloride for 6 min,which showed 30％ contamination rate; (2)In the initial medium (1/2MS + NAA 0.2 mg/L + 6-BA 0.6 mg/L),the buds germinated on the 8th day with the induction rate of 47％; (3)In the optimum subculture medium (1/2MS + IBA 0.3 mg/L + 6-BA 0.6 mg/L + white sugar 30 g/L),axillary buds were multiplied by 3.4 times on the 25th day; (4)The induction effect was the best with the rooting rate of 52％ in the rooting medium (1/4MS + IBA 0.15 mg/L + NAA 0.075 mg/L).[Conclusion]It was beneficial to the adventitious bud induction for Chinese fir with the initial medium (1/2MS + NAA 0.2 mg/L + 6-BA 0.6 mg/L) and the optimum subculture medium (1/2MS + IBA 0.3 mg/L + 6-BA 0.6 mg/L + white sugar 30 g/L).NAA was conducive to rooting in vitro,and rooting induction effect was the best in the rooting medium of 1/4MS + IBA 0.15 mg/L + NAA 0.075 mg/L.关键词
杉木/组织培养/不定芽/植株再生/条件筛选Key words
Cunninghamia lanceolata/ tissue culture / adventitious bud/ plant regeneration / condition selection分类
农业科技引用本文复制引用
莫雅芳,戴勤,谭玲,苏治南,卢亮,杨梅..杉木茎段不定芽诱导及植株再生条件筛选[J].南方农业学报,2013,44(5):810-814,5.基金项目
国家自然科学基金项目(31070560) （31070560）
广西大学实验室建设与实验教学改革资助项目(20121602) （20121602）
