工业微生物2013，Vol.43Issue(3)：55-60,6.DOI:10.3969/j.issn.1001-6678.2013.03.011
重组大肠杆菌发酵生产谷氨酰胺转胺酶的过程优化
Process optimization of transglutaminase fermentation by recombinant Escherichia coli
摘要
Abstract
The yield of Streptomyces hygroscopicus pro-transglutaminase (pro-TGase) of recombinant Escherichia coli carrying pBB1-1010 by using fed-batch fermentation in 3 L fermenter was investigated.The recombinant pro-TGase was transformed into mature TGase by dispase treatment.When the fed-batch strategy was performed in the TB medium containing 8 g/L of glycerol,the highest OD600 value of the cells was 43.0.The extracellular TGase yield and productivity reached 12.71 U/mL and 0.18 U/mL/h respectively.In the case of fed-batch fermentation using optimized bacterial medium,the extracellular TGase yield and productivity were respectively increased to 15.68 U/mL and 0.37 U/mL/h by addition of 150 mmol/L glycine and 20 mmol/L CaCl2 after induction.Meantime,a large amount of the intracellular pro-TGase was accumulated,and consequently the total pro-TGase yield reached 30.35 U/mL which meant the recombinant strain had the potential for industrial application.关键词
谷氨酰胺转胺酶/大肠杆菌/补料分批发酵Key words
transglutaminase/ Escherichia coli / fed-batch fermentation引用本文复制引用
马建龙,刘松,陈康康,张东旭,张娟,堵国成,陈坚..重组大肠杆菌发酵生产谷氨酰胺转胺酶的过程优化[J].工业微生物,2013,43(3):55-60,6.基金项目
863项目(2011AA100905) （2011AA100905）
国家自然科学基金(编号:31000031,31171639) （编号:31000031,31171639）
江苏省自然科学基金. （）
