工业微生物2013，Vol.43Issue(3)：72-76,5.DOI:10.3969/j.issn.1001-6678.2013.03.014
乙醇脱氢酶基因和乙醛脱氢酶基因的克隆及表达
Cloning and expression of adh2 and aldh6
汤斌 1杨亚平 1张莹莹1
作者信息
- 1. 安徽工程大学微生物发酵安徽省工程技术研究中心,安徽芜湖241000
- 折叠
摘要
Abstract
In order to realize ethanol directly transform to acetic acid,the alcohol dehydrogenase 2 (adh2) and acetaldehyde dehydrogenase 6 (aldh6) genes were cloned and expressed.The genomic DNA of Saccharomyces cerevisiae as template was extracted and adh2 and aldh6 genes were obtained by PCR.Two genes were inserted into expression vector pYES2.Then the expression vectors pYES2-adh2,pYES2-aldh6 and pYES2-aldh6-adh2 were constructed.They were transformed to INVScl.Sequence tests showed that the acquired fragments exhibited 96％ and 99％ homology to adh2 and aldh6 gene sequences from GenBank report.Then these recombinants were grown on glucose and shift to inductive medium.The activities reached to the highest when the recombinants were induced for 4 hours.The activities of adh2 and aldh6 were 2.7-fold and 2-fold higher than those of the wild types,respectively.关键词
乙醇脱氢酶/乙醛脱氢酶/克隆/表达Key words
alcohol dehydrogenase 2 / acetaldehyde dehydrogenase 6 / clone/ expression引用本文复制引用
汤斌,杨亚平,张莹莹..乙醇脱氢酶基因和乙醛脱氢酶基因的克隆及表达[J].工业微生物,2013,43(3):72-76,5.
