环境工程学报2013，Vol.7Issue(7)：2788-2794,7.
降解聚丁二酸丁二醇酯菌株的诱变选育及降解性能
Mutation breeding of poly(butylene succinate)-degrading strain and its degradability
摘要
Abstract
In order to improve the degradability of the a preserved PBS-degrading strain,five different mutagenic methods were compared.The strain DSCF-8 which could degrade PBS film was chosen as the starting strain for mutagenesis.By analyzing the morphological features and mitochondrial cytb sequence,strain DSCF-8 was identified as Fusarium oxysporum.It was indicated that the effects of combined mutation were stronger than that of single mutation,and generation rate of efficient mutant strains reached 4.55％ and 1.30％,respectively.However,the transmissibility of those mutant strains were not stable after subculturing.Mutation induced by using single mutagen diethyl sulfate was much more effective than by ultraviolet and microwave,and the mutant strain D8B14 obtained was stable after subculturing.The optimal conditions were suggested as 5％ diethyl sulfate with a trea ting time of 40 min.The degradation rate of mutant strain D8B14 was 34.49％ higher than that of the starting strain,and the enzyme activity was improved by 23.86％.In addition,the degradability of this mutant strain was much higher at 35℃,and the pH tolerance became much stronger.关键词
聚丁二酸丁二醇酯(PBS)/尖孢镰刀菌/降解性能/诱变/酶活性Key words
poly (butylene succinate) (PBS) / F.oxysporum / degradability/ mutagenesis / enzyme activity分类
资源环境引用本文复制引用
董骞,梁英梅,田呈明,梅雪立..降解聚丁二酸丁二醇酯菌株的诱变选育及降解性能[J].环境工程学报,2013,7(7):2788-2794,7.基金项目
教育部留学回国基金和科技部林业微生物菌种保藏平台项目(2005DKA21207-8) （2005DKA21207-8）
