华南农业大学学报2013，Vol.34Issue(2)：167-176,10.
玉米根际溶磷细菌的分离、筛选及溶磷能力研究
A Study on the Isolation, Screening and Phosphate Solubilizing Capacity of Phosphate Solubilizing Bacteria in the Rhizosphere of Corn
摘要
Abstract
Phosphate-solubilizing bacteria were isolated from the rhizosphere of corn, and 38 strains of inorganic phosphate-solubilizing bacteria and 35 strains of organic phosphate-solubilizing bacteria were screened by using traditional method of isolation and culture of microorganisms. The number of both total bacteria and phosphate-solubilizing bacteria in the rhizosphere soil was higher than that in the bulk soil. Major bacterial colony was milky or yellow, irregular or roughly round, smooth, thick, flat, non-transparent with ragged edge. Furhter study on phosphorus solubilizing capacity of the strains by Mo-Sb colori-metry method indicated that pH was significantly correlated with the content of available P of inorganic phosphate-solubilizing bacteria strains, but not correlated with that of organic phosphate-solubilizing bacteria strains. There were great differences between solubilizing calcium phosphate by inorganic phosphate-solubilizing bacteria and solubilizing lecithin by organic phosphate-solubilizing bacteria. Their phosphate-solubilizing capacity ranged from 8.88 to 108.31 and 0.51 to 3.53 mg/L, respectively. The strain SWJ1-4 and SWJ3-1 had a higher ability in solubilizing inorganic phosphate than other strains and strain RYJ1-6 had a higher capacity in solubilizing organic phosphate. The three strains had great potential in further development of microbiological fertilizer because of their stronger phosphate-solubilizing ability, faster growth rate and better growing state.关键词
溶磷菌/溶磷能力/根际/玉米Key words
phosphate-solubilizing bacteria/ phosphate-solubilizing ability/ rhizosphere/ corn分类
农业科技引用本文复制引用
白文娟,胡蓉蓉,章家恩,冯丽芳,徐华勤..玉米根际溶磷细菌的分离、筛选及溶磷能力研究[J].华南农业大学学报,2013,34(2):167-176,10.基金项目
广东省科技计划项目(2006B20601004) （2006B20601004）
广东省自然科学基金(8151064201000048,9451064201003801,S2011010001570) （8151064201000048,9451064201003801,S2011010001570）
中国博士后特别资助(201003355) （201003355）
广东省教育部产学研结合项目(2010B090400453) （2010B090400453）
