华南农业大学学报2013，Vol.34Issue(2)：224-229,6.
猪精原干细胞体外培养条件的优化
Optimizing the Culture Conditions of Porcine Spermatogonial Stem Cells (PSSCs) in vitro
摘要
Abstract
The testicular cells of Landrace piglets aged from 1 to 5 days were isolated by two-step enzymatic digestion method. The Percoll discontinuous density gradients method was used to purify the porcine spermatogonial stem cells ( PSSCs). The effects of glial cell-line derived neurotrophic factor ( GD-NF) and leukemia inhibitory factor (LIF) on PSSCs proliferation were investigated by MTT method. The results showed that the method for PSSCs isolation and purification were effective. By means of MTT assay , the results indicated that LIF and GDNF factor could promote PSSCs proliferation, and the combination of 20 ng o mL-1 GDNF and 1 000 U o mL-1 LIF showed the strongest effects.关键词
猪/精原干细胞/体外培养Key words
porcine/ spermatogonial stem cells ( PSSCs) / in vitro culture分类
生物科学引用本文复制引用
师如意,卫恒习,李莉,白银山,张秀娟,吴珍芳,张守全..猪精原干细胞体外培养条件的优化[J].华南农业大学学报,2013,34(2):224-229,6.基金项目
国家973计划(2011CB944202,2010CB945001,2009CB941601) （2011CB944202,2010CB945001,2009CB941601）
国家科技支撑计划(2011BAD19B03) （2011BAD19B03）
