华南农业大学学报2013，Vol.34Issue(3)：287-291,5.
亚洲栽培稻与短花药野生稻种间杂交障碍观察
Observations on Interspecific Hybridization Barriers Between Oryza sativa and O.brachyantha
摘要
Abstract
Oryza sativa and O.brachyantha belong to AA and FF genomes in genus Oryza,respectively.The interspecific hybridization barrier limits the transfer of elite genes from O.brachyantha into O.sativa.By using technique of whole-mount eosin B-staining confocal laser scanning microscopy,the development of hybrid embryo and endosperm after crossing between O.sativa and O.brachyantha and embryo sac abortion process of interspecific hybrid F1 were observed.The results showed that there was a high double-fertilization ratio of 82.93％ appearing in the pollinated spikelets,but hybrid embryo ceased to develop and began to degenerate at the globular-shaped stage,meanwhile,free endosperm nuclei degenerated before forming cellular endosperm.It induced hybrid embryo abortion and the matured hybrid seed failed be produced.During the process of hybrid embryo sac development,hybrid megasporocyte finished meioses,but abnormal tetrad was formed which induced abnormal functional megaspores.Embryo sac was degenerated at the stage of mono-nucleate and two-nucleate embryo sac,therefore,no normal embryo sac was formed and hybrid was highly sterile.关键词
亚洲栽培稻/短花药野生稻/细胞学观察/种间杂交障碍Key words
Oryza sativa/ O.brachyantha / cytological observation / interspecific hybridization barrier分类
农业科技引用本文复制引用
傅雪琳,刘向东,卢永根..亚洲栽培稻与短花药野生稻种间杂交障碍观察[J].华南农业大学学报,2013,34(3):287-291,5.基金项目
广东省自然科学基金(9151064201000067) （9151064201000067）
公益性行业(农业)科研专项(201003021) （农业）
广东省自然科学基金(021037) （021037）
