核农学报2013，Vol.27Issue(6)：867-872,6.
外源NO对NaCl胁迫下山葡萄叶片叶绿素荧光和抗氧化酶活性的影响
Effects of Exogenous Nitric Oxide on Chlorophyll Fluorescence and Antioxidant Enzymes Activity in Amur Grape (Vitis Amurensis Rupr) Leaves under Salt Stress
摘要
Abstract
To evaluate the role of nitric oxide (NO) in plant salt tolerance,this study was conducted in nutrient solution to investigate the effects of exogenous sodium nitroprusside (SNP),an NO donor,on chlorophyll content,chlorophyll fluorescence parameters and antioxidant enzymes activity in Vitis amurensis ‘ Shuangfeng' 1-year cutting seedling under 50 mmol· L-1 NaCl stress.The results showed that exogenous NO significantly promoted the activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in Vitis amurensis leavs,which led to the decrease of malondialdehyde (MDA),thus protected chloroplast memberance stability and increased chlorophyll content.On the other hand,exogenous NO increased photochemical efficiency of photosystem Ⅱ(F,/Fm),actual photochemical efficiency (ΦPSⅡ),photochemical quenching coefficient (qP) and regulated thermal energy dissipation (ΦNPQ)under NaCl stress.However,the minimal fluorescence (Fo) and decreased photo damage (ΦNO) in Vitis amurensis leaves were decreased.These results indicated that NO enhanced the tolerance of Vitis amurensis seedlings to NaCl stress through the protecting mechanism of thermal dissipation which alleviated photo inhibition caused by salt stress,improving photochemical electron transport efficiency and maintaining the normal function of PSⅡ to some extent.关键词
山葡萄/盐胁迫/一氧化氮/叶绿素荧光/抗氧化酶Key words
Amur grape/Salt stress/Nitric oxide/Chlorophyll fluorescence/Antioxidant enzymes引用本文复制引用
赵滢,艾军,王振兴,秦红艳,张庆田,许培磊,刘迎雪,沈育杰..外源NO对NaCl胁迫下山葡萄叶片叶绿素荧光和抗氧化酶活性的影响[J].核农学报,2013,27(6):867-872,6.基金项目
现代农业产业技术体系建设专项资金(农业部、财政部nycytx-30-01) （农业部、财政部nycytx-30-01）
山葡萄种质资源收集、编目与利用(农业部NB2011-2130135-37) （农业部NB2011-2130135-37）
